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Dietary calcium phosphate content and oat -glucan influence gastrointestinal microbiota, butyrate-producing bacteria and butyrate fermentation in weaned pigs

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The following antibodies were used: anti-insulin (SigmaAldrich, #I2018, 1:400), anti-insulin (Boster, #BM0080, 1:100), anti-C-peptide (DSHB, #GN-ID4-S, 1:50), anti-C-peptide (Cell Signaling Technology, 4593S, 1:200), anti-ZnT8 (Thermo Fisher Scientific, #PA5-103252, 1:400), anti-ZnT8 (Lifespan Biosciences, #LS-C296473, 1:400), anti-Glucagon (Cell Signaling Technology, #2760S, 1:100), anti-ARX (R&D Systems, #AF-7068-SP, 1:50), anti-NKX6.1 (DSHB, #F55A12-c, 1:50), anti-GFP (MilliporeSigma, #06-896, 1:500), anti-mCherry (Abcam, #ab125096, 1:500), anti-CXCR4-PE (Thermo Fisher Scientific, #MHCXCR404, 1:100), anti-CD117-APC (Thermo Fisher Scientific, #CD11705, 1:100), anti-PDX1 (R&D Systems, #AF2419, 1:100), anti-somatostatin (Santa Cruz Biotechnology, #sc-7819, 1:100), anti-ATF4 (Proteintech, #10835-1-AP, 1:1000), anti-PERK (Cell Signaling Technology, #3192S, 1:1000), anti-p-PERK (ABclonal, #AP1501, 1:1000), anti-eIF2 (Beyotime, #AF6771, 1:1000), anti-p-eIF2 (Beyotime, #AF1237, 1:1000), anti--actin (Beyotime, #AF5001, 1:5000), anti-GAPDH (Beyotime, #AF1186, 1:5000), antibodies conjugated with Alexa Fluor 488 (715-545-150, 711-545-152, 712-545-150, 703-545-155, 1:500), Alexa Fluor 594 (715-585-150, 711-585-152, 712-585-153, 1:500), and Alexa Fluor 647 (715-605-151, 711-605-152, 712-605-153, 705-605-003, 713-605-147, 1:500) from Jackson ImmunoResearch, anti-mouse IgG HRP-linked antibody (Cell Signaling Technology, #7076P2, 1:10,000), and anti-rabbit IgG HRP-linked antibody (Cell Signaling Technology, #7074S, 1:10,000)

However, the muscle growth only occurs when combined with rigorous physical training

Both approaches enhance wound healing through different mechanisms