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[DOI] [PubMed] [Google Scholar] 59.Collins D.A., Hogenkamp H.P., OConnor M.K., Naylor S., Benson L.M., Hardyman T.J., Thorson L.M

The pellet was treated with permeabilization buffer and vortexed for 10 min at 4 with consistent mixing, followed by centrifugation at 15,000 g for 45 min at 4

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In the context of PMOS, the oxidative stress connection is directly relevant: studies confirm that granulosa cells from PMOS patients exhibit obvious inflammation and oxidative stress, with significantly reduced proliferation and increased apoptosis