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Lehrer and Leavis, 1978), whose efficiency can be evaluated using Stern-Volmer plot (named after Otto Stern (18881969) and Max Volmer (18851965) who proposed this approach in 1919) correlating changes in fluorescence intensity with the concentration of quencher, and whose slope gives the Stern-Volmer constant, K SV , a quantitative measure of fluorescence quenching efficiency (Stern and Volmer, 1919)