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The lysate was clarified by centrifugation at 15,000 nx g for 15 min, protein concentrations were determined using the BCA total protein assay (Pierce), and then appropriate volumes of the lysates were diluted with 2x reducing Laemmli buffer and heated for ten min at 95 C

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Here, the consumption of BP led to the greatest increase in ex vivo proliferation of lymphocytes, while these results could simply be related to the presence of a great concentration of vitamins, trace elements, and amino acids that stimulate differentiation and proliferation of immune cells (161, 163)

REDEFINE 1, in adults with overweight or obesity without diabetes, reported 22.7% mean weight loss for the combination at 68 weeks on the on-treatment analysis, with 60% of combination participants reaching at least 20% loss and 23% reaching at least 30% (Aronne et al., 2025)

Data analysis and quantitation was performed using LabSolutions software (ver