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Despiste the wide variability in maturation timelines and functional benchmarks across different iHLO differentiation protocols 10,15,16,29,30,31 , our findings contribute a valuable reference for guiding hepatic organoid development, especially for optimizing their use in preclinical research and tailored applications such as drug metabolism and toxicology

Understanding these differences prevents cross-contamination of protocols

As detected by DCFH-DA staining, GCLC overexpression reestablished cellular ROS levels in cells expressing shACTL6A, phenotypes remained significant when treated with H 2 O 2 and was reversed by NAC (Fig

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