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Interestingly, for HQ we found structures (within the lowest 1 kcal/mol) involving the peripheral site, which for KA is about 4.1 kcal/mol above the best-bound minima (Fig

Recently, it has been found that physiological doses of CO, produced during the HO-1 enzymatic reaction, can act as intracellular second messengers, promoting mitochondrial regeneration and restoring mitochondrial membrane potential, thereby reducing mitochondrial ROS production (Huang et al., 2018)

Material and methods Cell culture Human hepatocellular carcinoma (HepG2) (ATCC, HB-8065) were maintained in DMEM (4.5 g/l glucose, pyruvate) (Gibco), supplemented with 10% fetal bovine serum (PAA Laboratories) and penicillin/streptomycin (25 g/ml each, Gibco) at 37 C, 5% CO 2

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