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Laboratory assays of oxidative status markers One oxidative damage (protein carbonyls) and two antioxidant (GPX and SOD) blood-based markers were assessed in whole blood samples using commercially available kits commonly applied to mammals 48,49 following the manufacturers instructions unless otherwise mentioned

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By disrupting the FOXO4-p53 interaction, FOXO4-DRI, a specific FOXO4 blocker, selectively induced p53 nuclear exclusion and apoptosis in senescent Leydig cells

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