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For data that passed normality tests, the Students t -test was used when differences between two groups were analyzed, and analysis of variance was used when differences between three or more groups were compared followed by the appropriate post hoc test

NIH/3T3 cells were stained with 0.5 M Calcein-AM and the fluorescence was detected by CLSM

Maintaining adequate levels can help reduce the mortality risk associated with these conditions

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