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Upon activation of the intrinsic apoptotic pathway, expression of Bcl-2 is suppressed while that of Bax increases, leading to the opening of the permeability transition pores (PTP), enhanced mitochondrial membrane permeability, loss of mitochondrial membrane potential, and the release of apoptotic proteins such as cytochrome C (Cyt-c) and DIABLO/Smac into the cytosol