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GHK-Cu GHK-Cu is a copper-bound tripeptide (Gly-His-Lys with Cu) referenced in extracellular-matrix and copper-transport research literature

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At the end of incubation, 20 L of MTS reagent was added to each well and incubated for another two hours, then analyzed with a microplate reader using a test wavelength of 490 nm within 10 min