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The retention of each sample was calculated using the formula: Cellular uptake of SPc-loaded fluorescent LNT The FITC (0.1 mg) was incubated with LNT (50 mg) activated by glutaraldehyde at room temperature for 12 h, followed by dialysis using a 20000 Da dialysis bag for 24 h to remove unbound FITC, yielding FITC-LNT