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We further generated a tdTomato reporter gene within FAPCAR mRNA to observe transfection

5 Translational perspectives 5.1 Preclinical models and biomarker development The preclinical models and biomarker development represent the cornerstone of translational research towards incorporation of natural supplements in breast cancer therapy by adding mechanistic insights, validating efficacy, predicting therapeutic response, relevance towards patients for personalized therapy as well as on addressing their safety and dosing issues (219)

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Cold stress in mice has also been shown to change the composition of the gut microbiota, and fecal transplants from cold-stressed donors to room temperature-housed recipients elicit the same increases in intestinal morphology, independent of food intake 5,7