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1 Urinary frequency is often the first symptom of IC to arise, but patients may not recognize it as a problem until they have endured many years of it.5 Among patients with IC, 92% report urinary frequency: 86% say that they urinate more than 11 times during a 24-hour period and 71% say that they urinate 3 or more times at nightthe definition of nocturia

[DOI] [PubMed] [Google Scholar] 124.Laron Z

Journal of Clinical Pharmacy and Therapeutics

Even if youre purifying the same protein over and over, it is probably still a good idea to clean and regenerate your column about every five purifications, or earlier if you notice that the binding capacity of your beads is getting worse